Dr. Sebastian van de Linde

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Dr. Sebastian van de Linde
Telefon:
E-Mail:
+49 931 31-80935
sebastian.vandelinde@uni-wuerzburg.de

Single-molecule photoswitching and super-resolution imaging


List of publications

2015 [to top]

  • Hennig, S., van de Linde, S., Lummer, M., Simonis, M., Huser, T., Sauer, M.: Instant Live-Cell Super-Resolution Imaging of Cellular Structures by Nanoinjection of Fluorescent Probes. Nano Lett. 15, 1374--1381 (2015).
     
  • Hennig, S., van de Linde, S., Bergmann, S., Huser, T., Sauer, M.: Quantitative Super-Resolution Microscopy of Nanopipette-Deposited Fluorescent Patterns. ACS Nano. 9, 8122--8130 (2015).
     
  • Wäldchen, S., Lehmann, J., Klein, T., van de Linde, S., Sauer, M.: Light-induced cell damage in live-cell super-resolution microscopy. Scientific Reports. 5, 15348-- (2015).
     
  • Laine, R.F., Albecka, A., van de Linde, S., Rees, E.J., Crump, C.M., Kaminski, C.F.: Structural analysis of herpes simplex virus by optical super-resolution imaging. Nat Commun. 6, (2015).
     

2014 [to top]

  • Ehmann, N., van de Linde, S., Alon, A., Ljaschenko, D., Keung, X.Z., Holm, T., Rings, A., DiAntonio, A., Hallermann, S., Ashery, U., Heckmann, M., Sauer, M., Kittel, R.J.: Quantitative super-resolution imaging of Bruchpilot distinguishes active zone states. Nat Commun. 5, (2014).
     
  • Mehlitz, A., Karunakaran, K., Herweg, J.-A., Krohne, G., van de Linde, S., Rieck, E., Sauer, M., Rudel, T.: The chlamydial organism Simkania negevensis forms ER vacuole contact sites and inhibits ER-stress. Cellular Microbiology. n/a--n/a (2014).
     
  • Löschberger, A., Franke, C., Krohne, G., van de Linde, S., Sauer, M.: Correlative super-resolution fluorescence and electron microscopy of the nuclear pore complex with molecular resolution. Journal of Cell Science. 127, 4351-4355 (2014).
     
  • Holm, T., Klein, T., Löschberger, A., Klamp, T., Wiebusch, G., van de Linde, S., Sauer, M.: A Blueprint for Cost-Efficient Localization Microscopy. ChemPhysChem. 15, 651--654 (2014).
     
  • Proppert, S., Wolter, S., Holm, T., Klein, T., van de Linde, S., Sauer, M.: Cubic B-spline calibration for 3D super-resolution measurements using astigmatic imaging. Opt. Express. 22, 10304--10316 (2014).
     
  • van de Linde, S., Sauer, M.: How to switch a fluorophore: from undesired blinking to controlled photoswitching. Chem. Soc. Rev. 2014. 43(4), 1076-1087 (2014).
     

2013 [to top]

  • Schäfer, P., van de Linde, S., Lehmann, J., Sauer, M., Doose, S.: Methylene blue- and thiol-based oxygen depletion for super-resolution imaging. Analytical Chemistry. 85, 3393 - 3400 (2013).
     
  • van de Linde, S., Aufmkolk, S., Franke, C., Holm, T., Klein, T., Löschberger, A., Proppert, S., Wolter, S., Sauer, M.: Investigating Cellular Structures at the Nanoscale with Organic Fluorophores. Chemistry & Biology. 20, 8 - 18 (2013).
     

2012 [to top]

  • Löschberger, A., van de Linde, S., Dabauvalle, M.-C., Rieger, B., Heilemann, M., Krohne, G., Sauer, M.: Super-resolution imaging visualizes the eightfold symmetry of gp210 proteins around the nuclear pore complex and resolves the central channel with nanometer resolution. Journal of Cell Science. 125, 570-575 (2012).
     
  • Klein, T., van de Linde, S., Sauer, M.: Live-Cell Super-Resolution Imaging Goes Multicolor. ChemBioChem. 13, 1861--1863 (2012).
     
  • Bar-On, D., Wolter, S., van de Linde, S., Heilemann, M., Nudelman, G., Nachliel, E., Gutman, M., Sauer, M., Ashery, U.: Super-resolution imaging reveals the internal architecture of nano-sized syntaxin clusters. Journal of Biological Chemistry. (2012).
     
  • Wolter, S., Loschberger, A., Holm, T., Aufmkolk, S., Dabauvalle, M.-C., van de Linde, S., Sauer, M.: rapidSTORM: accurate, fast open-source software for localization microscopy. Nat Meth. 9, 1040--1041 (2012).
     
  • van de Linde, S., Heilemann, M., Sauer, M.: Live-Cell Super-Resolution Imaging with Synthetic Fluorophores. Annual Review of Physical Chemistry. 63, (2012).
     

2011 [to top]

  • Klein, T., Loeschberger, A., Proppert, S., Wolter, S., van de Linde, S., Sauer, M.: Live-cell dSTORM with SNAP-tag fusion proteins. NATURE METHODS. 8, 7-9 (2011).
     
  • Wolter, S., Endesfelder, U., van de Linde, S., Heilemann, M., Sauer, M.: Measuring localization performance of super-resolution algorithms on very active samples. Optics Express. 19, 7020--33 (2011).
     
  • Kaminski Schierle, G.S., van de Linde, S., Erdelyi, M., Esbjörner, E.K., Klein, T., Rees, E., Bertoncini, C.W., Dobson, C.M., Sauer, M., Kaminski, C.F.: In Situ Measurements of the Formation and Morphology of Intracellular β-Amyloid Fibrils by Super-Resolution Fluorescence Imaging. Journal of the American Chemical Society. 133, 12902-12905 (2011).
     
  • van de Linde, S., Wolter, S., Sauer, M.: Single-molecule Photoswitching and Localization. Aust. J. Chem. 64, 503--511 (2011).
     
  • van de Linde, S., Loschberger, A., Klein, T., Heidbreder, M., Wolter, S., Heilemann, M., Sauer, M.: Direct stochastic optical reconstruction microscopy with standard fluorescent probes. Nat. Protocols. 6, 991--1009 (2011).
     
  • van de Linde, S., Krstic, I., Prisner, T., Doose, S., Heilemann, M., Sauer, M.: Photoinduced formation of reversible dye radicals and their impact on super-resolution imaging. Photochem. Photobiol. Sci. 10, 499-506 (2011).
     

2010 [to top]

  • Uphoff, S., Holden, S.J., Reste, L.L., Periz, J., van de Linde, S., Heilemann, M., Kapanidis, A.N.: Monitoring multiple distances within a single molecule using switchable FRET. NATURE METHODS. 7, 831-U90 (2010).
     
  • Heidbreder, M., Endesfelder, U., van de Linde, S., Hennig, S., Widera, D., Kaltschmidt, B., Kaltschmidt, C., Heilemann, M.: Subdiffraction fluorescence imaging of biomolecular structure and distributions with quantum dots. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH. 1803, 1224-1229 (2010).
     
  • Wombacher, R., Heidbreder, M., van de Linde, S., Sheetz, M.P., Heilemann, M., Cornish, V.W., Sauer, M.: Live-cell super-resolution imaging with trimethoprim conjugates. NATURE METHODS. 7, 717-U72 (2010).
     
  • Kottke, T., van de Linde, S., Sauer, M., Kakorin, S., Heilemann, M.: Identification of the Product of Photoswitching of an Oxazine Fluorophore Using Fourier Transform Infrared Difference Spectroscopy. The Journal of Physical Chemistry Letters. 1, 3156-3159 (2010).
     
  • Endesfelder, U., van de Linde, S., Wolter, S., Sauer, M., Heilemann, M.: Subdiffraction-Resolution Fluorescence Microscopy of Myosin-Actin Motility. CHEMPHYSCHEM. 11, 836-840 (2010).
     
  • Wolter, S., Schuettpelz, M., Tscherepanow, M., van de Linde, S., Heilemann, M., Sauer, M.: Real-time computation of subdiffraction-resolution fluorescence images. JOURNAL OF MICROSCOPY-OXFORD. 237, 12-22 (2010).
     
  • van de Linde, S., Wolter, S., Heilemann, M., Sauer, M.: The effect of photoswitching kinetics and labeling densities on super-resolution fluorescence imaging. JOURNAL OF BIOTECHNOLOGY. 149, 260-266 (2010).
     

2009 [to top]

  • Heilemann, M., van de Linde, S., Mukherjee, A., Sauer, M.: Super-Resolution Imaging with Small Organic Fluorophores. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION. 48, 6903-6908 (2009).
     
  • van de Linde, S., Endesfelder, U., Mukherjee, A., Schuettpelz, M., Wiebusch, G., Wolter, S., Heilemann, M., Sauer, M.: Multicolor photoswitching microscopy for subdiffraction-resolution fluorescence imaging. PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES. 8, 465-469 (2009).
     
  • Hennig, S., van de Linde, S., Heilemann, M., Sauer, M.: Quantum Dot Triexciton Imaging with Three-Dimensional Subdiffraction Resolution. NANO LETTERS. 9, 2466-2470 (2009).
     

2008 [to top]

  • Heilemann, M., van de Linde, S., Schuttpelz, M., Kasper, R., Seefeldt, B., Mukherjee, A., Tinnefeld, P., Sauer, M.: Subdiffraction-resolution fluorescence imaging with conventional fluorescent probes. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION. 47, 6172-6176 (2008).
     
  • van de Linde, S., Kasper, R., Heilemann, M., Sauer, M.: Photoswitching microscopy with standard fluorophores. APPLIED PHYSICS B-LASERS AND OPTICS. 93, 725-731 (2008).
     
  • van de Linde, S., Sauer, M., Heilemann, M.: Subdiffraction-resolution fluorescence imaging of proteins in the mitochondrial inner membrane with photoswitchable fluorophores. JOURNAL OF STRUCTURAL BIOLOGY. 164, 250-254 (2008).