Lehrstuhl für Biotechnologie und Biophysik

    Confocal Laser Scanning Microscope (CLSM) with spectral detector

    The Nikon C1 is a standard confocal microscope with 3 separate single channel detectors (PMTs). Additionally the emission light can be spectrally resolved by switching to a spectral detector. Using 32 PMTs the spectral resolution can be varied between 2.5, 5 and 10nm. Resolving the spectrum of the emission enables to correct for scattering of tissue, autofluorescence or split overlapping emission spectra of dyes.

    Microscope-body: Nikon TiE
    Objective: 100x Oil, NA 1.49, Apo-TIRF
    100x Siliconoil, NA 1.3
    Laser: 405nm, 488nm, 561nm, 639nm
    Detection:3 PMTs
    Spectral Detector, 32 Channels, spectral resolution 2.5/5/10nm

    Spectral separation of SiR700-Tubulin (Magenta) and SiR-Actin (Cyan) using 640nm excitation

    Emissionspectra of SiR700-Tubulin (Magenta) and SiR-Actin (Cyan)

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    Lehrstuhl für Biotechnologie und Biophysik
    Am Hubland
    97074 Würzburg

    Tel.: +49 931 31-84507
    E-Mail

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    Hubland Süd, Geb. B1 Hubland Nord, Geb. 32 Julius-von-Sachs-Platz 2 Fabrikschleichach Hubland Süd, Geb. B2 Campus Medizin