Re-scan Confocal Microscopy (RCM, under construction)
Reference: de Luca et al., 2013
We set up a correlative system using the super-resolution technique dSTORM and Re-scan Confocal Microscopy (RCM), which is resolution-enhanced form of LSM. By re-scanning the emission light after the pinhole, RCM gains a resolution improvement of 1.4 fold. This will increase the lateral resolution from 240 nm of a confocal microscope to 170 nm of the RCM, while maintaining the sectioning capability of a confocal system. Since the detector (Photomultiplier Tube) is replaced by a sCMOS camera with high quantum efficiency, the signal-to-noise ratio of the RCM is twice as good as for a confocal microscope. With this system we are now capable of quantitatively investigating protein distributions in the context of dynamic processes with high spatial resolution.