Lehrstuhl für Biotechnologie und Biophysik

    Direct Stochastic Optical Reconstruction Microscopy (dSTORM)

    Reference: van de Linde et al., 2011

    Olympus setup (2D, dual-color)

    Microscope-body: Olympus IX-71 equipped with nosepiece stage (IX2-NPS; Olympus), inverted
    Objective: Olympus 60x oil, NA 1.45 PlanApo, TIRF
    Laser: 405 nm, 488 nm, 532 nm, 568 nm: 100 mW
    642 nm: 200 mW
    Cameras: 2 EMCCD, Andor iXon DU-897

    High Power setup (2D, dual-color)

    Microscope-body: Olympus IX-71 equipped with nosepiece stage (IX2-NPS; Olympus), inverted
    Objective: Olympus APON 60XO TIRF oil, NA 1.49
    Laser: 405 nm, 488 nm: 200 mW
    514 nm, 558 nm: 500 mW
    642 nm: 1000 mW
    Cameras: 2 EMCCD Andor iXon Ultra DU-897

    3D setup (2D, 3D, dual-color)

    Microscope-body: Zeiss Axio Observer.Z1
    Objective: Zeiss 63× LD C-Apochromat NA 1.15 water-immersion, 600 µm working distance
    Zeiss Plan-Apochromat 100x NA 1,46 oil
    Laser: 405 nm, 488 nm: 200 mW
    532 nm: 500 mW
    640 nm: 250 mW
    Cameras: 2 EMCCD Andor iXon Ultra DU-897
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    Lehrstuhl für Biotechnologie und Biophysik
    Am Hubland
    97074 Würzburg

    Tel.: +49 931 31-84507
    E-Mail

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    Hubland Süd, Geb. B1 Hubland Nord, Geb. 32 Julius-von-Sachs-Platz 2 Fabrikschleichach Hubland Süd, Geb. B2 Campus Medizin