Collaborative Research Centre / Transregio 166
High-end light microscopy elucidates membrane receptor function
In the CRC/TRR 166 ReceptorLight high-end light microscopy techniques with highest spatial and time resolution are applied and further developed to gain deeper insight into the function of membrane receptors. The participating groups in Jena and Würzburg bundle their methodological expertise in the field of super-resolution microscopy, electrophysiology, and biophysics of membrane receptors to generate new insights into the function and distribution of diverse membrane receptors, and in parallel, to induce the development of new high-end light-microscopy methods.
“ReceptorLight Symposium 1“, June 7-9. 2017, Tagungszentrum Juliusspital, Würzburg
This symposium will cover relevant topics from all fields of high-end light microscopy and its usability to study the function of membrane receptors.
For more informations see Symposium_ReceptorLight.pdf
Quantification of membrane receptors: From single receptors to clinical applications
The Receptome harbors an uncharted number of therapeutic applications in health and disease. It comprises the entirety of receptor molecules in an organism and accounts for more than 5% of all proteins. The drug-receptor concept, which exploits the highly specific interaction between diffusible ligands and receptors, has led to the discovery and development of a large number of drugs that target specific receptors. However, despite their central therapeutic relevance, knowledge on specific receptor parameters, particularly for low abundant receptors remains elusive; this includes numbers, different functional states in response to different external stimuli, their distribution and interactions, as well as their operating modes.
In close collaboration with other departments and the university of Jena we aim to investigate receptors at all levels, from molecular mechanisms and functions, to cellular interactions and communication in both healthy and diseased model organisms. Together with the university hospital, we will translate our findings to clinical applications, therein improving current molecular diagnostics and personalized therapies.
The development of super-resolution microscopy has opened a window for the study of cellular processes at a nanomenter scale; it is now possible to investigate quantitatively how receptors are organized and how they interact at the molecular level. From such investigations, we are able to improve our knowledge of the relations between receptor localization, receptor abundance, and receptor functionality, which is key to understanding how alterations of malfunction of any of these parameters can lead to diverse diseases.