Lehrstuhl für Biotechnologie und Biophysik

    Photometry unlocks 3D information from 2D localization microscopy data

    23.11.2016

    Franke C., Sauer M., van de Linde S.

    Nature Methods (2016)

    doi:10.1038/nmeth.4073

    We developed a straightforward photometric method, temporal, radial-aperture-based intensity estimation (TRABI), that allows users to extract 3D information from existing 2D localization microscopy data. TRABI uses the accurate determination of photon numbers in different regions of the emission pattern of single emitters to generate a z-dependent photometric parameter. This method can determine fluorophore positions up to 600 nm from the focal plane and can be combined with biplane detection to further improve axial localization.

    Zurück zu: Startseite

    Kontakt
    Biotechnologie & Biophysik
    Biozentrum
    Am Hubland
    97074 Würzburg

    Tel.: +49 931 31-84507
    Fax: +49 931 31-84509

    Suche Ansprechpartner
    Hubland Süd Hubland Nord Campus Dallenberg Fabrikschleichach Humangenetik Campus Medizin